SigE is a chaperone for the Salmonella enterica serovar Typhimurium invasion protein SigD.

نویسندگان

  • K H Darwin
  • L S Robinson
  • V L Miller
چکیده

SigD is translocated into eucaryotic cells by a type III secretion system. In this work, evidence that the putative chaperone SigE directly interacts with SigD is presented. A bacterial two-hybrid system demonstrated that SigE can interact with itself and SigD. In addition, SigD was specifically copurified with SigE-His(6) on a nickel column.

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

ثبت نام

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

منابع مشابه

مقایسه پلی‌مورفیسم ژنومی و ارتباط ژنتیکی سویه‌های بالینی سالمونلا انتریکا سرووار تیفی موریوم در استان کرمان به روش ERIC- PCR و Box-PCR

Introduction: Salmonella is one of the most important causes of gastroenteritis in humans. Salmonella enterica Serovar Typhimurium has many hosts in addition to humans, and its prevalence in the community is high. The aim of the study was comparing the genetic diversity of Salmonella enterica serovar Typhimurium isolated from human fecal samples by both of ERIC-PCR and BOX-PCR method. Methods:...

متن کامل

Multilocus Sequence Typing of the Clinical Isolates of Salmonella Enterica Serovar Typhimurium in Tehran Hospitals

Background: Salmonella enterica serovar Typhimurium is one of the most important serovars of Salmonella enterica and is associated with human salmonellosis worldwide. Many epidemiological studies have focused on the characteristics of Salmonella Typhimurium in many countries as well as in Asia. This study was conducted to investigate the genetic characteristics of Salmonella Typhimurium using m...

متن کامل

Effect of Electron Beam Irradiation on Survival of Escherichia coli O157:H7 and Salmonella enterica serovar Thyphimurium in Minced Camel Meat during Refrigerated Storage

Background: Electron beam irradiation is one of the effective ways to control food-borne pathogens. We evaluated the effect of electron beam irradiation on survival of Escherichia coli O157:H7 and Salmonella enterica serovar Thyphimurium in minced camel meat during refrigerated storage. Methods: The meat samples were inoculated with E. coli O157:H7 and S. enterica serovar Thyphimurium and then...

متن کامل

The DnaK/DnaJ chaperone machinery of Salmonella enterica serovar Typhimurium is essential for invasion of epithelial cells and survival within macrophages, leading to systemic infection.

Salmonella enterica serovar Typhimurium, similar to various facultative intracellular pathogens, has been shown to respond to the hostile conditions inside macrophages of the host organism by inducing stress proteins, such as DnaK. DnaK forms a chaperone machinery with the cochaperones DnaJ and GrpE. To elucidate the role of the DnaK chaperone machinery in the pathogenesis of S. enterica serova...

متن کامل

Molecular Detection of Salmonella enterica Serovar Typhimurium in Ready-to-Eat Vegetable Salads Consumed in Restaurants of Tabriz, North-West of Iran

Background: In recent years, food-borne outbreaks have been increased by consumption of raw fruits and vegetables contaminated with bacterial pathogens like Salmonella spp. in many countries. This study was designed in order to molecular detection of Salmonella in Ready-to-Eat Vegetable Salad (REVS) consumed in restaurants of Tabriz, North-West of Iran. Methods: In this cross-sectional study, ...

متن کامل

ذخیره در منابع من


  با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

ثبت نام

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

عنوان ژورنال:
  • Journal of bacteriology

دوره 183 4  شماره 

صفحات  -

تاریخ انتشار 2001